Phenotypic and genotypic characterization of staphylococcus aureus

by k.s.v.prasad.
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Staphylococcus identified from the skin surface of different individuals were characterized by their antimicrobial susceptibility and random amplified polymorphic DNA.

The development of antimicrobial resistance nearly has followed the therapeutic use of antimicrobial agents Since antibiotic use became wide spread 50 years ago, bacteria have developed resistance. RAPD typing provide a more rapid and reliable method to distinguish between high and low virulence S.aureus strains.

The RAPD molecular size ranges 0.3-5 Kbp. Staphylococcus strains are of great clinical importance since they are often resistance towards a variety of antibiotics besides their resistance to beta lactams.

By means of restriction endonuclease digests and DNA hybridization studies five staphylococcal strains were analyzed for their plasmid similarities and plasmid associated resistance determinants.

We found that strains isolated at our laboratory contained contained identical or at least most similar plasmids. Resistance determinants for gentamycin is chromosomally encoded where as resistance to chloramphenicol was plasmid associated.

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